FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6674063
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76630.11481761559637661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG35850.016.45498369
AGGATAG99300.015.2165269
CCTATAC11200.015.0894753
GTATAGG10000.014.8321311
GTATAAT16950.014.2450331
GTATTAT26900.014.2333081
GGATAGT81700.014.0186669
GTATTAG22300.013.9210761
AGAGGAT175800.013.91290769
TATACCG8450.013.87803655
GTGTAGA34100.013.75862469
GTATATA18350.013.7221421
CCCTATA7350.013.1383472
GTATAAC10850.013.0343471
GAGGATA134250.013.00249569
TTATACT16250.012.9472944
TATACAG17700.012.86105255
AAGAGGA247400.012.67519669
TAGTGTA46250.012.60650668
GTATTAA27600.012.4975831
Produced by FastQC (version 0.11.2)