FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341616
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83980.11438898465950821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA22900.014.7609251
TATACAG18300.014.5110045
AGGATAG98200.014.08861869
AGTGTAG39350.013.67777269
GGATAGT79100.013.25966669
GAGGATA132150.013.13216469
TATAATA33250.013.0682972
AGAGGAT173550.013.06099369
GTGTAGA36450.012.96756869
GTATTAT30700.012.5835341
CTATACT14850.012.5405424
GTATTAA33400.012.0826551
AAGAGGA242600.011.96026269
CGAAGAG310500.011.8337869
GTATTAG19850.011.642261
GAAGAGG286900.011.5926369
GTATAAG14250.011.376431
ATCTAGG20050.011.3540951
TAGTGTA49000.011.33668368
TTTATAC20250.011.2398873
Produced by FastQC (version 0.11.2)