FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357842
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108200.10446191397783437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA59200.024.41874769
AGTGTAG79900.022.8854969
TAGTGTA102700.017.80563568
ATAGTGT134800.016.2267169
GGATAGT217350.015.55598669
AGGATAG278950.015.32413869
GAGGATA366500.014.72291869
GATAGTG182550.014.72267569
AGAGGAT478300.014.25337369
GTCTAGG14750.013.5627411
GTATAAA19900.013.5192631
TATAATA28050.013.4024492
AAGAGGA644450.013.38387569
GAAGAGG795350.013.200026569
GTATTAA31450.013.1604921
TAGGCCG7050.012.720135
ATATAGA25100.012.3674551
TCTAGGG11100.012.1180322
CGAAGAG913750.012.11639569
TATACAG16200.011.9228575
Produced by FastQC (version 0.11.2)