FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928747
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122630.12351004613170222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG55600.017.37312169
GTGTAGA49450.015.9060869
TAGTGTA68250.014.3560368
ATAGTGT87150.014.012979569
GTATTAA39700.013.2921391
GTATTAT39050.013.2484231
GATAGTG120250.013.13938469
TATACAG21100.013.0770645
TTATACT22050.012.9826534
GGATAGT127000.012.87565369
AGGATAG154850.012.81006369
CGAAGAG467850.012.74182869
GAAGAGG423250.012.37284169
GTATATA23500.012.32836151
TAGACAG19950.012.2749055
AGAGGAT256200.011.84944969
TATAATA37350.011.8196482
TATTATA37300.011.7430282
AAGAGGA353400.011.62625569
GTATAAA23450.011.6192521
Produced by FastQC (version 0.11.2)