FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10010758
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122670.12253817343302076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA59250.022.3002569
AGTGTAG75500.020.65336269
TAGTGTA93450.016.6877468
ATAGTGT125200.015.45816269
TATACAG18500.014.1689985
GTATAGA13650.014.1503721
AGGATAG230400.013.730494569
GATAGTG167750.013.42919469
GGATAGT187450.013.23252569
GTATAGG14800.013.050851
GTATAAT22200.013.0508491
AAGAGGA509550.012.89078669
GAGGATA295600.012.55761469
TTATACT20050.012.3853674
AGAGGAT377150.012.34863669
GAAGAGG609250.012.30448669
GTATTAT35550.012.2248461
TTAGGGT13100.012.1109254
TATAATA33850.011.9207862
TAGGCCG7550.011.8774635
Produced by FastQC (version 0.11.2)