FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7695672
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80770.10495509683884656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA55550.031.61079469
AGTGTAG78300.024.14462769
TAGTGTA101000.018.68548668
ATAGTGT128550.014.78877167
AGGATAG247750.014.565310569
GGATAGT195750.013.65845769
GAGGATA317400.013.608150569
GATAGTG164550.013.54366969
TATACGG7950.013.4482685
AGAGGAT401700.012.95952669
TTACATA12350.012.5662614
AAGAGGA525200.012.44761669
GTATAGA10350.012.3297121
GAAGAGG626900.011.58942269
ATCTAGG17650.011.5291911
TACATAG14450.011.4563215
GTATTAT23200.011.4470681
TATACTG15800.011.3505795
GTATAGT16200.011.2837241
GGACCGT13600.010.905246
Produced by FastQC (version 0.11.2)