FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360788
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115360.11134288241396312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA48950.019.31168269
AGTGTAG59000.019.12132669
TAGTGTA74600.015.4004768
GAGGATA237950.015.06440969
AGGATAG177100.014.80530269
GGATAGT139650.014.15584969
AGAGGAT317550.013.9500369
ATAGTGT94700.013.40664569
TATACAG20500.012.9545635
GTATTAA37300.012.8531231
GAAGAGG535100.012.77237869
GATAGTG126100.012.69477969
AAGAGGA439300.012.59695169
GTATTAT34750.011.9104771
CGAAGAG596500.011.80469669
GTATTAG26250.011.6940081
TTATACT20700.011.496034
GTATAAT19950.011.4104761
TATAATA34200.011.3947472
GTATAAG20100.011.3253221
Produced by FastQC (version 0.11.2)