FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9587715
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99750.10403938790420866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA91500.034.23608469
GGTGTAG161600.022.2243369
AGGTGTA179950.019.90116368
TGTAGAT53350.017.1368469
GAGGTGT224250.015.9550267
AGAGGTG272100.013.16303966
GTATTAA26850.012.3321761
ATAGAGA531000.011.77938569
AATAGAG638250.011.70811469
GTATAAT16850.011.6677681
GAGAGGT307700.011.61792865
GTATAAA20800.011.441911
GTATAGA15400.011.4225281
TATACAG18550.011.1553675
TAGAGAG439750.011.03059169
AGAGAGG381500.010.87903669
GAATAGA766800.010.65865169
GTATATG17150.010.4580811
ATATTAG20050.010.1496351
TAATACT31250.010.0432084
Produced by FastQC (version 0.11.2)