FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51847985
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA254300.015.05886769
AATAGAG1597050.014.08460869
ATAGAGA1279400.013.98433669
TAGAGAG1008800.013.71371269
GAGAGGT648800.013.62335269
GTATTAA176500.013.3861051
GAATAGA2019650.013.09679569
AGAGAGG876800.013.03970169
AGGTGTA352700.012.57998568
GAGGTGT456050.012.48208569
AGAGGTG595350.012.29670369
TATACAG100850.012.2431065
GTATAAT125450.012.2348231
GTATATA106100.012.0280361
TATAATA176050.011.9297052
GTATTAT180650.011.6275361
CGAATAG2330800.011.48609469
TTATACT105350.011.3270834
GTATTAG142950.011.0265691
GTATAAA124350.010.8175071
Produced by FastQC (version 0.11.2)