FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339759
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202350.46627013159025654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA39950.031.34763569
GGTGTAG67000.019.66996269
AGGTGTA73400.017.95569868
TGTAGAT24500.015.34883369
GAGGTGT94200.014.02806967
GTATAGA6650.014.0022171
TATACAG9050.012.9565715
TACACCG7350.012.66881755
GGACCGT7650.012.6242726
GTATAGG5750.012.5952321
GTGTAAG6650.012.4464151
GTCCTAG9450.012.4080011
GTGTACA8900.012.0123061
AGAGGTG113150.011.77098666
GTCCTAT7350.011.7302511
GTACTAC10950.011.6531231
TTATACT9600.011.1364084
GACTAGA7450.011.1098881
GTATTAT13150.010.7525831
ATAGTAC10100.010.5851013
Produced by FastQC (version 0.11.2)