FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10916555
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159600.14619996876303926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA70400.025.14089469
AGGTGTA122400.016.85697668
GGTGTAG134750.015.46479469
TAGAGAG348350.014.05408469
ATAGAGA435900.013.39212469
AGAGAGG290650.013.0930669
GAGGTGT159300.012.90934867
GAGAGGT221150.012.6367169
AATAGAG528850.012.47358669
GTATAGA13300.012.4476171
AGAGGTG198000.011.23907469
GAATAGA642150.011.21300369
GTATATA19400.011.2004481
TATAATA28300.010.8450232
TGTAGAT58550.010.72458469
GTATTAA33050.010.3314281
TATAGGG9750.010.2569992
GTATAAT21100.010.1345821
TTAGACA17350.09.9385574
CGAATAG726400.09.92728168
Produced by FastQC (version 0.11.2)