FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11240826
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA58300.015.56313969
GAGGTGT105000.014.94968769
AATAGAG390700.014.92290369
TAGAGAG240000.014.50407169
ATAGAGA305250.014.4778369
GAATAGA504450.014.34819969
GAGAGGT148650.014.11070469
GTATTAA44850.013.7647741
GTATTAT45100.013.6884741
AGAGAGG203000.013.34085569
CGAATAG603900.013.23072269
AGAGGTG135950.013.17037569
AGGTGTA81350.013.14755768
TATTATA41600.012.9323352
GTATAGA18150.012.7314161
TATACAG23850.012.7252155
GTATAAT26900.012.4364941
AATACCG31950.011.8738795
TTTATAC29900.011.6496083
CTAAGAG20550.011.5866426
Produced by FastQC (version 0.11.2)