FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32470099
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG389910.12008278755171026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG474800.015.47655169
GAGAGGT278950.015.19955369
ATAGAGA614050.014.7535469
AATAGAG782250.014.64632269
AGAGAGG398950.013.89641369
GAATAGA1004500.013.493896569
TATACAG72900.012.726325
CGAATAG1152550.012.20055969
GTATTAA138550.011.9243281
TATAATA128500.011.8889432
TATACGG41850.011.7847055
AGAGGTG261800.011.60944869
ATCTAGG73100.011.1824221
GTATTAT130300.011.1705071
TATTATA135000.011.1121512
TAGACAG63050.010.9948445
TTATACG43050.010.97532754
CTATACT68400.010.94142154
ACGAATA1417250.010.80791269
GTATTAG92650.010.7958661
Produced by FastQC (version 0.11.2)