FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924508
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99510.11150194498116871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA47000.014.09427869
GTATAGA17150.013.4747621
GAGAGGT109650.013.37272269
AGGTGTA61200.013.36159668
ATAGAGA208400.013.31060669
AATAGAG257750.013.17152169
GAATAGA325700.012.64812369
TAGAGAG166600.012.61193769
GAGGTGT81200.012.0245869
TATACAG21750.011.8928415
AGAGAGG148850.011.79800169
CGAATAG369050.011.62986469
GTATTAA34700.011.6296541
TTATACT20850.011.4133864
GTATTAC35500.011.3675781
AGAGGTG103350.011.15001669
CCTAGAT20300.010.873093
GTATATA25150.010.8342661
GTACTAT29750.010.7821761
GTATTAT36350.010.7222141
Produced by FastQC (version 0.11.2)