FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11383133
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125030.10983795058882295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA70550.029.7816469
GGTGTAG128850.017.53820869
AGGTGTA128400.017.43937568
TAGAGAG399550.014.50662969
AGAGAGG326100.014.494328569
ATAGAGA513950.014.34537769
AATAGAG646750.014.13103169
GAGGTGT163750.013.9056469
GAATAGA814350.013.595242569
GAGAGGT243150.013.59313369
CCTATAC16300.013.1181913
GTATTAT40550.013.0131331
GTATTAA46100.012.568681
AGAGGTG210750.012.47428769
TTATACT27200.012.2990824
GTATTAC34150.012.1191421
AATACCG30600.012.0595825
GTATAAG21300.011.9821141
GTATATA30000.011.9562061
CGAATAG960750.011.87550569
Produced by FastQC (version 0.11.2)