FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698177
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG137000.014.45472269
AATAGAG219200.014.29103769
ATAGAGA176000.014.15281869
GTATAGA22600.013.5813561
AGAGAGG120050.013.21947169
GAGAGGT84600.013.17196669
TATACAG23600.012.8602065
GAATAGA277950.011.91579369
GTATTAA42750.011.5361641
CTATACT21350.011.4691444
CCTATAC16900.011.4278063
CGAATAG311000.011.39274769
GTATAAT26200.011.3203191
AGAGGTG82150.011.29700169
TATACCG12350.011.170445
GTATTAG28700.010.9350581
TAATACT49650.010.8361714
AGTCGCG17600.010.77928512
TAGACAG17600.010.581775
ATCTAGG25750.010.5806271
Produced by FastQC (version 0.11.2)