FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39177250
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463690.11835695461013726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGT416350.014.38508869
TAGAGAG655350.014.02433669
AATAGAG1033000.013.81677269
ATAGAGA829800.013.39179269
GAATAGA1310150.013.08223469
AGAGAGG568450.012.86056569
GTATTAA169350.012.8110671
GTATATA113000.012.5148391
TATACAG96200.012.0821035
GTATAAT111200.011.9729831
AGAGGTG388150.011.91040769
GTGTAGA182400.011.87835269
GAGGTGT304000.011.77999669
GTATTAT175400.011.7792351
CGAATAG1507000.011.64123569
GTATTAG121000.011.51637651
CTATACT78900.011.3214244
TATAATA172750.011.2196652
TTATACT93150.011.1445334
AGTCGCG74300.010.91004812
Produced by FastQC (version 0.11.2)