FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21479709
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG256650.11948485894292143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA120000.020.0103969
AGGTGTA189400.016.0121168
GTATAAT57200.012.9642231
TATAATA87550.012.9593472
GTATTAA87050.012.9167391
GGTGTAG244900.012.76341669
GAGGTGT240950.012.60110367
AATAGAG709100.012.59656369
GAATAGA888450.012.49627669
GTATAGA33650.012.2998621
TTATACT50400.012.0434474
GTATTAT87200.011.826571
CGAATAG1030350.011.81665169
ATAGAGA583700.011.74453169
GAGAGGT332450.011.67491469
GTATATA55150.011.6324591
AGAGGTG301300.011.58801169
TAGAGAG477850.011.54474669
GTATAAA56150.011.4867161
CTATACT39700.011.4670614
Produced by FastQC (version 0.11.2)