FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA30450.014.61538669
AGAGAGG101950.014.34783869
GAGAGGT74650.014.326488569
TAGAGAG123350.014.15204669
GAGGTGT51150.014.02896469
GTATATA16800.013.7556751
GTATAGA10800.013.4134951
ATAGAGA154400.013.29466969
AATAGAG190350.013.21242469
TATACCG8450.013.0613125
TATACAG16000.012.7181985
GAATAGA240050.012.67578269
GTATAAA19950.012.1024021
AGAGGTG66150.012.09946169
GTATAGG11050.011.8614481
CCTATAC10600.011.7135073
GTCTTAG12800.011.5871041
AGGTGTA42250.011.350668
CTATACT13450.011.2829834
TATAGTG13450.011.2829835
Produced by FastQC (version 0.11.2)