FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9898812
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGAG364200.012.87404869
CCAGAGA293450.012.86230669
GGATCTC59050.012.73714969
AGAGGAT132300.012.64787469
GACCAGA440400.012.40135769
AGAGAGG194150.012.33268669
GTATTAA32200.012.2167861
CAGAGAG240400.012.12712669
GATCTCG47050.011.95263369
GTATAAT19850.011.821061
ATCTAGG21950.011.7905691
GTATAGA14050.011.5432881
GAGGATC100000.011.38520267
AGGATCT82100.011.01010868
GTATTAC28750.010.9222361
GTATAAA19100.010.8399161
GTATTAG23250.010.8344761
AGACCAG508200.010.75360268
TTAGACA19000.010.7147034
TAATACT36950.010.6456484
Produced by FastQC (version 0.11.2)