FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6104863
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTAC65300.013.47253869
TATTATA27200.013.065832
CTCTCTA76650.013.00792869
GTATTAT28350.012.9017911
ACCTCTC110700.012.84020869
ATTATAC17200.012.8386763
TATACTG13600.012.6851675
GTATAGA10550.012.4287741
TAGACAG11900.012.1777615
GACCTCT134950.011.811131569
GTATATA17700.011.6970181
CTCTACA54800.011.458117569
GTATAAA17200.011.4351951
TACTATA18900.011.3187532
CCTCTCT100500.011.22545969
GTATAAG13700.011.0822841
AGACCTC161500.011.04435769
GTCTAGT15450.010.9437141
GTATTAA28500.010.8966951
GTATAAT18400.010.8769551
Produced by FastQC (version 0.11.2)