FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5655845
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG27150.020.33193869
AGGCATG154800.017.27266769
TGATCTC40450.016.88788469
ACTAGGC316950.015.9033369
GCATGAT85900.015.7040769
GGCATGA115300.015.6495269
TAGGCAT195250.015.40827669
TATACAG12550.015.3963945
CTAGGCA245650.015.28061169
GTATAAG13500.014.05859851
GACTAGG397100.013.76207469
GTCCTAA8650.013.5636281
CCTATAC7200.013.4182653
CTATACT12750.013.2604044
TAGACAG8050.012.85878855
TATACCG6000.012.651625
GTATAAT18300.012.6338821
TAGATAG24050.012.6253175
GTATAGA10400.012.6084981
TATAATA37550.012.588942
Produced by FastQC (version 0.11.2)