FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6573863
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG21300.031.10088769
CTATCTC51500.017.82073469
CCTATCT58800.015.72541268
GTATTAT20200.015.5452371
ACGGACT217550.013.65511169
GACGGAC273000.013.63672169
GTATTAG17300.013.3639871
AGACGGC12550.013.19619469
TATACCG4254.984031E-1012.9902815
TCCTATC72850.012.83465867
CGGACTC174350.012.3089169
ATCTCGT17400.011.8974369
TATTATA23250.011.8728382
TATACAG15250.011.7657875
CTCCTAT91350.011.25517668
AGACGGA332950.011.25370168
GGACTCC155800.011.11699669
ACTCCTA105650.010.90757469
GTATTAA18850.010.8006031
GACTCCT137600.010.78204669
Produced by FastQC (version 0.11.2)