FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5894052
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGAGC48900.014.39317269
GTATAGA11000.014.1173941
CGCTAAG4650.014.0968258
CTATACT14400.013.41914
CTCCTGA84850.013.41829469
TACTATA18800.013.3988212
GTATAGT18000.013.2285211
GTATATA15650.012.7893481
TCCTGAG67050.012.761169
TATACTG13350.012.6652185
TATTATA27550.012.6503142
GACTCCT128450.012.62405969
GTATTAT28800.012.5814741
TATAATA25250.012.5726832
GTATAAT16800.012.5301211
TATACAG15450.012.5071225
GTATTAA27250.012.2840031
TAGGCCG5651.8189894E-1212.2146045
GTATTAG19400.012.0960031
ATAATAC33550.011.9306293
Produced by FastQC (version 0.11.2)