FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4693162
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG18200.016.30236469
GTATAGA7950.014.75832751
TATAGAG7000.014.2960492
CTAGGGG3202.7528586E-814.0187543
GGCAGAA81050.013.79162769
GTATTAC22600.013.7422991
TATACTG12150.013.3485185
AGGCAGA103600.013.28729769
GTATATA14050.013.263031
CAGGCAG125400.013.15085269
GACAGGC179400.013.01936369
TTAGACA9300.012.986774
GTATAAA14100.012.7265171
TATAGAC11950.012.4169992
ACAGGCA156200.012.41305869
AAATCTC27700.012.205969
CTATACT10200.012.1791894
GTATATG9950.012.1386481
TAATACT23050.011.97664454
GTCCTAA6350.011.955681
Produced by FastQC (version 0.11.2)