FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12766305
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG34100.014.0654685
CTATACT31500.014.021464
GACGTAG251500.013.85557369
ACGTAGA206350.013.7605769
TAGAGGA127250.013.3397769
CGTAGAG166200.013.32738969
GTATAGA26400.013.0710951
TACTATA41900.012.4352652
AGACGTA301100.012.249207569
GTATTAA61950.011.9760351
GTAGAGG152150.011.88229769
TAATACT69800.011.6667634
GTATATA42050.011.6530131
TATTATA60750.011.6439322
GTATAAT35950.011.4225741
GTATAGG22100.011.3984691
GTATTAT63450.011.257831
TATACTG32650.011.0968555
TATAATA61350.011.0801022
ATAATAC66700.011.070873
Produced by FastQC (version 0.11.2)