FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10528617
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG8050.015.0023065
CAAGAGG199900.013.617669
GTATTAT50850.013.5722471
AGAGGCA123600.012.89614369
AAGAGGC149600.012.66126569
GTATTAA54700.012.5538931
ACAAGAG259800.012.33709569
TATAATA49900.012.1008852
TTATACT28200.011.991094
TATTATA51900.011.8340212
GTATTAG37150.011.796621
TATACCG14350.011.5418435
AGACAAG354000.011.50045769
GTATAAA35000.011.4367491
GTATAAT33550.011.4167671
ATTATAC33200.011.3284423
GTCTAGG15600.011.2812761
TAGATAG44850.011.2324965
GACAAGA312400.011.19858869
CTATACT26350.011.1306384
Produced by FastQC (version 0.11.2)