FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039934
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCT76150.016.17425769
CCGAGGC94700.015.04617669
TATACAG11550.014.6386315
ACCGAGG124500.014.57613769
GTATTAT23400.014.1581461
TCTATAC9400.013.9491063
TATAATA20550.013.6009152
GTATTAA19550.013.5923631
GTATAAT16800.013.5576891
GACCGAG155400.013.34287769
TACTATA15750.013.1451542
CTATACT12850.012.8892834
GTATTAG16150.012.8212311
TATACGG6800.012.6857885
GTATAGG9850.012.61296751
AGGCTGA53600.012.48714169
AATACCG16200.012.1407695
GTATATG14600.012.0550311
TATTATA23500.012.0404012
TAAGGGT8600.012.0368674
Produced by FastQC (version 0.11.2)