FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5531960
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT32700.018.25301669
CTACGCT66400.016.00364569
CGCTACG96650.014.92143869
GCTACGC77200.014.79268569
TATAATA24200.014.6857932
CGCTATC24950.014.51961269
TACGCTA49800.014.06380969
GTATTAT25300.014.048551
TATTATA23700.013.97652
TCTATAC8500.013.8017943
CTATACT13100.013.6964374
TACTATA16900.013.4751242
TATCTCG16550.013.1334569
GTACTAT22100.013.1160131
ACGCTAC132100.013.08492369
GACGCTA150100.012.98685669
GTATATA14450.012.8955741
ATAATAC26550.012.8660783
TATACAG13500.012.7793235
GTATTAG17150.012.6762631
Produced by FastQC (version 0.11.2)