FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6740180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA10350.015.6694161
TATACCG9150.014.3296395
GTATTAA30250.013.9164921
AGAGAGG100600.012.62052769
TATACGG9900.012.5470055
CAGAGAG125800.012.50581169
GTATATA16300.012.4899221
ATTATAC20150.012.3290653
CCAGAGA147950.012.312510569
TATTATA28050.012.3009852
ACCAGAG186550.012.298552569
TAGACAG13200.012.2856085
GTATTAT28450.012.1286651
TTATACG10850.012.0844424
GTATTAG22550.011.93557551
AGACCAG262850.011.81305269
ATAATAC31350.011.6665133
TATAGGG8000.011.6451882
TATAATA27000.011.6292142
GACCAGA219750.011.61797669
Produced by FastQC (version 0.11.2)