FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7236257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG46200.021.80688969
AGATCTC63450.018.0534169
ACTAGAT125850.015.07864469
GTATAGG10600.014.3239461
CGTACTA245900.014.26971369
CCGTACT314950.013.54034369
TAGATCT86750.013.323868
GTATAGA9850.013.312611
GTACTAG217100.013.28615369
TACTAGA165650.013.226269
CTAGATC93550.013.16670869
ACCGTAC410200.012.59995569
TATACAG15300.012.178515
TTATACT13950.011.8730384
CCTAGAT19050.011.7737063
GACCGTA458800.011.75407269
ATCCTAG21850.011.6868131
GTATTAT21200.011.556821
TAGACAG14400.011.5019265
AATACCG18900.011.5019265
Produced by FastQC (version 0.11.2)