FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234312
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG14300.028.95319269
GTATAGA2655.456968E-1219.5306191
TAAGGTG2153.1468517E-919.25815
GAATCTC23450.018.98008269
CGCGAAT5400.018.52665915
AGGCGAA77450.017.9083669
ATCTAGG5600.017.8681951
ATCCTAG5850.017.6944081
AATACCG5100.017.5903225
CTAAGAG4200.017.24973126
AAGGCGA106450.017.24317269
CTGTCCG2451.5896148E-816.8973189
GAGTCGC4900.016.89731811
TTAGACA2251.10771E-716.8682924
CCTAGAT6800.016.744263
GCCGTGT4750.016.7046332
GTATAAT4550.016.68331
AGTCGCG4800.016.53025212
TATACGG2301.4225589E-716.5019595
TAAGACG2351.817516E-716.1504924
Produced by FastQC (version 0.11.2)