FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6674063
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA155450.015.66946669
ACTAGGC197300.015.47591869
GTATAGA11500.014.4029671
CCTATAC11700.014.1551713
AGGCATG109450.014.05915869
GACTAGG248900.013.95869469
TAGGCCG4700.013.9482185
TAGGCAT125950.013.83354369
GTATTAA29900.013.3873731
GAGTCGC13350.013.17982411
TGATCTC40200.013.13123569
GATCTCG29450.012.76972269
GGCATGA87500.012.73604969
GTATTAG21450.012.7089111
GTATATA18550.012.6495071
AGAGCCG14450.012.17624129
AGTCGCG12850.012.08166612
AATACCG20300.012.0677225
GTATAAA20100.012.0174011
TAATAGT29000.012.0166434
Produced by FastQC (version 0.11.2)