FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341616
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG18550.018.2306965
ACCTCTC194100.014.52247569
CCTATAC12750.014.07373
CTCTCTA125400.013.75677469
GACCTCT243300.013.59942769
CCTCTCT164750.013.50760769
GTATAAT19650.013.3473711
TCTCTAC105050.013.10451669
GTATAGA13250.013.02263451
TATACTG16850.012.9021385
TTATACT17450.012.6561794
CATCTCG37200.012.52088369
TAGACAG13100.012.3807695
AGACCTC295500.012.1778669
GTATTAG21850.011.84553051
TTTATAC22800.011.8052423
GTATATG15400.011.652721
GTCTAGG11000.011.60790351
GTATAAA20300.011.5599941
GTATAGG11150.011.4517431
Produced by FastQC (version 0.11.2)