FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357842
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG51800.024.31110269
AAATCTC88900.018.16291669
GAAATCT112200.014.60636868
AGGCAGA380000.014.43627269
CAGGCAG471400.014.3891669
ACAGGCA599600.014.38533269
GGCAGAA300150.014.34556969
GACAGGC737500.014.17496969
TATACGG10800.014.0571645
GCAGAAA246300.013.75588769
TAGACAG15850.013.7145025
CAGAAAT194900.013.48915369
GTATTAT28450.013.0989181
GTATTAA32800.012.9397291
GTATAGG12850.012.889381
CCTATAC13900.012.4114923
AATACCG27400.012.3408285
GTATAGA13250.012.2398451
GTATAAA21150.012.2361521
AGACAGG907550.012.23367869
Produced by FastQC (version 0.11.2)