FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928747
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG45900.015.40924469
GTATAGG15050.014.4443051
AGATCTC62450.014.41943469
GACCGTA357000.014.34186969
GTATTAA38250.014.2533941
TATACAG20250.014.1423515
ACTAGAT113800.013.73396869
AGACCGT443250.013.38812669
GTATATA22250.013.337091
GTATTAT37400.013.1011481
CCGTACT235300.012.81532569
GTATAAT25600.012.6701141
ACCGTAC320000.012.4098269
CGTACTA187350.012.13589569
TATTATA38400.012.1302272
ATATAAT45400.011.8566321
CCTATAC16600.011.84773353
GTATAGA14300.011.8236791
GAGACCG532000.011.78374769
TACTATA26100.011.7656492
Produced by FastQC (version 0.11.2)