FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10010758
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG47900.022.40040669
GAATCTC75800.016.1125869
GGCGAAT146500.014.97788669
ACTAAGG438700.014.76140369
TAAGGCG273000.013.68663969
GACTAAG544000.013.62914269
GCGAATC111900.013.4735669
CTAAGGC346450.013.46378569
AGGCGAA183200.013.23917369
AAGGCGA242300.013.04286769
CGAATCT99950.012.97871668
GTATAAA21400.012.9009011
TATACAG20400.012.855355
TATACCG11550.012.5478045
TCTATAC12150.012.495983
TATAATA31650.012.3196592
GTATTAT36400.012.2301611
TTTATAC23550.012.161333
AGACTAA662950.011.89669969
GTATTAA37350.011.826691
Produced by FastQC (version 0.11.2)