FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7695672
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG46600.033.9095769
CTATCTC77350.021.32113369
CCTATCT97850.017.06593568
AGACGGC22500.014.56745869
CTCCTAT152650.014.19402469
TCCTATC122200.013.91915167
ACTCCTA190650.013.64511569
GACTCCT259700.013.59084269
CGGACTC382350.013.57154969
GGACTCC316750.013.48486969
ACGGACT475300.013.43634969
GTATAAT14300.013.0311361
GTATTAC22350.012.8151921
TATAATA20350.012.7174132
GACGGAC581750.012.3773869
GTATTAA23800.012.3244211
TAGACAG13800.012.2522585
GTATTAT23300.012.1445781
GTCTTAG9550.011.924361
TATACCG9300.011.8731565
Produced by FastQC (version 0.11.2)