FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360788
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGAG236500.015.11355269
CCTGAGC180150.014.67007869
CTCCTGA308150.014.06259369
GACTCCT488100.013.83311469
GTATTAA35400.013.4520091
CTGAGCA146150.013.12544169
TGAGCAT114050.012.91726369
ACTCCTG430250.012.82228869
GTATAAT21400.012.7386861
AGACTCC580350.012.05040669
GTATAGA16050.011.8249411
GTATTAC32050.011.7357211
CTATACT18550.011.7188474
TATAATA31100.011.6497642
TAGACAG18450.011.5953975
GTATTAT32900.011.4325181
TTATACT21500.011.3948364
GTATAAA23400.011.3549741
GTATATA23050.011.2279811
TATACTG24300.011.0758915
Produced by FastQC (version 0.11.2)