FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9587715
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG78600.031.95667569
GAATCTC125650.022.29699169
GGAATCT169750.017.31757768
ATCTCGT45200.015.57200269
AGGAATC201700.015.37807667
GAGGAAT275250.012.83567166
CGTAGAG527950.011.89409369
ACGTAGA633150.011.8142369
GTATAGG13900.011.4194611
AGGAATG80250.011.30741869
CTATACT16500.011.0833134
TAGAGGA376100.010.96269569
GACGTAG750850.010.90888369
GTAGAGG447450.010.89558269
GTATAGA15050.010.7761581
TATAGGG11550.010.7546972
TATACCG10300.010.384955
GTATTAG20700.010.335321
AGAGGAA355700.010.15562265
TTAGGGT15250.09.95544054
Produced by FastQC (version 0.11.2)