FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51847985
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG171300.018.9125669
ACGCTAT416400.016.75376769
CGCTACG1186000.015.03709269
GCTACGC935200.014.70164369
TACGCTA597450.014.67388769
CTACGCT778200.014.39127769
ACGCTAC1582650.014.28992269
CTATCTC272400.013.85645969
GACGCTA1892850.013.84556969
CGCTATC327800.013.78809969
GCTATCT307250.012.73391468
TATAATA172800.012.4998772
GTATTAA182750.012.4617931
TATACAG106800.012.4383675
GTATAAT119750.012.2463711
TTATACT113400.011.9274434
GTATTAT187000.011.7726181
CTATACT99850.011.6454464
TATTATA181450.011.5997332
GTATAGA72950.011.5887011
Produced by FastQC (version 0.11.2)