FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339759
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG14450.022.68327969
GATCTCG39300.019.31435669
TATGCCG15200.019.29147143
CGTATGC18100.016.77274341
GGATCTC47600.016.30893568
CTCGTAT15100.016.22151639
TCGTATG16600.015.37902140
AAAGGGG21150.015.33442768
GTATGCC20100.015.27527342
CCGTCTT19100.014.26884347
GTACTAT9450.014.2420651
ATGCCGT20650.013.865944
TCTCGTA17400.013.68074938
GCCGTCT18750.013.61524546
TCTAGGC5850.013.5664773
GTACTAA8650.013.5644731
CGCGTAT5650.013.43334839
AGGATCT63450.012.94167167
TATACCG4154.84215E-912.4722065
CTAACGT3902.7279384E-812.3867834
Produced by FastQC (version 0.11.2)