FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10916555
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG74200.022.41221469
TACATCT132200.014.32783268
CTCTCTA347700.014.18969269
TCTCTAC282250.013.37288269
CCTCTCT448000.013.09989769
CTACATC149850.012.73215967
ACCTCTC526900.012.44133669
CTCTACA231350.011.94550869
ATCTCGT48550.011.938827569
GACCTCT629650.011.32616469
TCTACAT186850.011.31902469
TATACAG18050.011.0875645
TATAATA28250.010.6264012
TATAGAC20550.010.5782572
GTATTAA33750.010.5311711
GTCTAAC14350.010.340221
GTATATA18850.010.2515581
TATACCG12300.010.0991025
ATCTAGG23600.010.0890511
GTATAGA14900.09.9585341
Produced by FastQC (version 0.11.2)