FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11240826
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG43600.018.99223769
GTATTAA45000.014.9531081
AAGAGGC254200.013.74944969
TATAATA37250.013.4313652
ACAAGAG439350.013.35025969
CAAGAGG335450.013.29909669
GACAAGA548450.013.1606169
AGAGGCA208950.012.99522369
GTATAGA17000.012.9909351
TATACAG23600.012.5736965
GTATAAT25950.012.4997071
AGACAAG643900.012.46893569
AGGCAAT142500.012.39671169
TAGGCCG7850.012.3073595
GTATAAA26050.012.1867921
CTATACT20250.012.0978964
GAGGCAA175550.012.08719869
ATAATAC49050.012.0291143
TTATACT24150.011.7158344
GTATTAT45150.011.69345951
Produced by FastQC (version 0.11.2)