FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32470099
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCT455200.016.1291869
CCGAGGC574350.015.67858469
ACCGAGG747250.015.31519369
GACCGAG952100.013.98774369
AGGCTGA312550.012.86024969
TATACAG75200.012.5724465
GTATTAA139550.012.2401821
AGACCGA1156100.012.15519569
CTATACT64700.012.0528174
TATAATA127100.012.0266292
GTATTAT125700.011.7220911
GAGGCTG487450.011.6858469
GTATAAT82100.011.6005461
GAGACCG1345700.011.21433469
TATAGTG64550.011.0117935
TATTATA129200.010.9765292
TAGACAG60950.010.9262385
GGCTGAT255250.010.8675969
AATACCG94350.010.7886525
GTATAGA55800.010.6366961
Produced by FastQC (version 0.11.2)