FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924508
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT21400.013.7054551
CCTGAGC130750.013.45769269
GTATAGG14950.012.92513751
CTCCTGA215250.012.79092169
GTATAAA22450.012.7570441
TATAATA35500.012.7323722
GACTCCT322950.012.67043469
TCCTGAG169500.012.39623269
GTATTAA35400.012.3790911
GTATTAT34400.012.3377231
GTATAGA15950.012.3311191
TATAGAC21700.012.2432692
TATACAG21750.012.21526055
CTGAGCA116800.012.19970469
ATAATAC39200.012.0587133
TTATAGT24100.012.0262754
TGAGCAT94000.011.78200769
GTATTAG23450.011.6244541
TACTATA25850.011.6124852
ATTATAC23500.011.5991363
Produced by FastQC (version 0.11.2)