FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11383133
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG69150.027.74084569
TGATCTC106650.018.66603569
CTAGGCA496650.014.9634769
TAGGCAT394200.014.82633769
AGGCATG323200.014.667376569
ACTAGGC624750.014.57371369
GACTAGG786650.014.10933669
TATACAG22400.013.8635215
GCATGAT198150.012.72800569
GTATAGA17100.012.712841
ATGATCT159300.012.62664368
GGCATGA253800.012.61517869
CATGATC159950.012.53213767
GTCTAGG14500.012.3746641
TAGATAG47150.012.1480185
AATACCG29050.011.9964925
AGACTAG966400.011.93126869
TAATACT50800.011.7505354
GTATAAT26750.011.73858451
GTATTAG30500.011.652941
Produced by FastQC (version 0.11.2)