FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698177
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTC161750.015.400413569
ACGGACT210200.014.77233969
GGACTCC140250.014.2680569
ACTCCTA81000.013.37470269
GACTCCT117600.013.29016469
TATCTCG26350.012.83172169
GACGGAC260500.012.67487569
CTCCTAT70350.012.26070469
GTATAAT24500.012.1127711
TATAATA40550.011.8279762
CTATACT19550.011.6489274
TATTATA39450.011.3705862
TATACCG11300.011.298335
GTATTAA38900.011.2658841
GTATAGA19350.011.2349391
TAGACAG17300.011.1694785
ATAATAC44150.011.0979863
GAGTCGC19050.010.86637211
TTAGACA20400.010.8252664
AGACGGA318050.010.7502369
Produced by FastQC (version 0.11.2)