FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39177250
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA631300.014.422564569
GACCGTA1223800.013.72958469
CCGTACT808250.013.6981869
GTATTAT164800.013.1285021
ACCGTAC1131150.012.56044469
GATCTCG154200.012.55212969
GTATTAA164050.012.5364581
GTATAAT107450.012.5245761
GTATAGA67900.012.3492711
TATACAG96400.012.3486735
ACTAGAT364550.012.19929369
TATAATA165200.012.0516262
TACTAGA488450.012.03617269
AGACCGT1474800.011.92161769
GTATATA110200.011.89891
TATTATA172950.011.8906492
GTACTAG613100.011.84004369
GTATAAA106750.011.3137091
AGATCTC207300.011.16765869
TTATACT92000.011.1390034
Produced by FastQC (version 0.11.2)