FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21479709
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG87750.021.38941869
GAATCTC149750.015.80536269
GTATTAA87100.013.8267841
GTATTAT89550.013.7182381
GCGAATC198950.013.59590767
GGCGAAT253700.013.25962769
GACTAAG831400.013.25061669
ACTAAGG677100.013.2485369
GTATAAG38550.013.0690011
TATACAG49050.013.0142865
CGAATCT199700.012.80213168
TAAGGCG439950.012.42221569
CTAAGGC552600.012.36860769
AGGCGAA314850.012.33905469
GTATATA54650.012.3128441
GTATAGA33200.012.1607871
GTATAAT52550.012.08256151
TATAATA87350.012.048212
ATCTAGG50150.012.0415121
AGACTAA1026950.012.00413669
Produced by FastQC (version 0.11.2)